Originalarbeiten / Original papers
2023
Gabriel E. Wagner, Thomas Franz Paul Stanjek, Dirk Albrecht, Michaela Lipp, Susanna J. Dunachie, Esther Föderl-Höbenreich, Katharina Riedel, Anne Kohler, Ivo Steinmetz and Christian Kohler. Deciphering the human antibody response against Burkholderia pseudomallei during melioidosis using a comprehensive immunoproteome approach. Front. Immunol.Volume 14 - 2023 doi.org/10.3389/fimmu.2023.1294113
Stefan Weiss, Silva Holtfreter, Tanja C. Meyer, Frieder Schmiedeke, Clemens Cammann, Marcus Dörr, Stephan B. Felix, Hans J. Grabe,Georg Homuth, Christian Kohler, Cedric Mahncke, Stephan Michalik, Matthias Nauck, Nele Friedrich, Stefanie Samietz, Henry Völzke, Uwe Völker and Barbara M. Bröker. Toxin exposure and HLA alleles determine serum antibody binding to toxic shock syndrome toxin 1 (TSST-1) of Staphylococcus aureus. Front. Immunol., 04 September 2023, Volume 14 - 2023 | https://doi.org/10.3389/fimmu.2023.1229562
Kohler C, King J, Stacker L, Goller KV, Moritz J, Pohlmann A, Nath N, Tzvetkova A, Rieck M, Paraskevopoulou S, Beslic D, Hölzer M, Fuchs S, Ziemann J, Kaderali L, Beer M, Hübner NO, Becker K. Neighborhood Watch: Genomic epidemiology of SARS-CoV-2 variants circulating in a German federal state, Mecklenburg-Western Pomerania, in 2020-2022. Emerg Microbes Infect. 2023 Aug 10;2245916. PMID: 37585712 DOI: 10.1080/22221751.2023.2245916
Busch L, Hanuschik AM, Avlasevich Y, Darm K, Hochheiser EF, Kohler C, Idelevich EA, Becker K, Rotsch P, Landfester K, Darvin ME, Meinke MC, Keck CM, Kramer A, Zwicker P. Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms. Pharmaceutics . 2023 Feb 11;15(2):609. doi: 10.3390/pharmaceutics15020609.
2022
Wagner GE, Berner A, Lipp M, Kohler C, Assig K, Lichtenegger S, Saqib M, Müller E, Trinh TT, Gad AM, Söffing HH, Ehricht R, Laroucau K, Steinmetz I. Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics. J Clin Microbiol. 2022 Dec 21;e0123422. PMID: 36541753 doi: 10.1128/jcm.01234-22.
Al-Bakri AG, Dahabiyeh LA, Khalil E, Jaber D, Kamel G, Schleimer N, Kohler C, Becker K.Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants. Antibiotics (Basel). 2022 Nov 11;11(11):1607. doi: 10.3390/antibiotics11111607
A. Adeyanju, F. Schaumburg, A. Onayade, A. Akinyoola, T. Adeyemi, O. Ugbo, R. Köck, Y. Amusa, O. Lawal, T. Adeyanju, N. Torimiro, D. Akinpelu, D. Kolawole, C. Kohler, K. Becker. Local epidemiology of nosocomial Staphylococcus aureus infection in a Nigerian university teaching hospital. Antibiotics 2022 11(10):1372
K.V. Goller, J. Moritz, J. Ziemann, C. Kohler, K. Becker, N.O. Hübner, The CoMV-Gen Study Group. Differences in clinical presentations of Omicron-infections with the lineages BA.2 and BA.5 in Mecklenburg-Western Pomerania, Germany, between April and July 2022. Viruses 2022, 14 (9), 2033. https://doi.org/10.3390/v14092033
M. Hussain, C. Kohler, K. Becker. Enolase of Staphylococcus lugdunensis Is a Surface-Exposed Moonlighting Protein That Binds to Extracellular Matrix and the Plasminogen/Plasmin System. Front. Microbiol., 03 March 2022 | https://doi.org/10.3389/fmicb.2022.837297
2021
R. Hertel, K. Schöne, C. Mittelstädt , J. Meißner , N. Zschoche , M.Collignon , Christian Kohler , I. Friedrich , D. Schneider , M. Hoppert , R. Kuhn , I. Schwedt , P. Scholz , A. Poehlein , M. Martienssen , T. Ischebeck , R. Daniel , F.M. Commichau. Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution. Environ Microbiol Rep. 2021 Nov 16. doi: 10.1111/1758-2229.13022.
Phenn J, Pané-Farré J, Meukow N, Klein A, Troitzsch A, Tan P, Fuchs S, Wagner GE, Lichtenegger S, Steinmetz I, Kohler C. RegAB Homolog of Burkholderia pseudomallei is the Master Regulator of Redox Control and involved in Virulence. PLoS Pathog. 2021 May 28;17(5):e1009604. doi: 10.1371/journal.ppat.1009604. PMID: 34048488
Tanja C. Meyer, Stephan Michalik,Silva Holtfreter, Stefan Weiss, Nele Friedrich, Henry Völzke, Thomas Kocher, Christian Kohler, Frank Schmidt, Barbara M. Bröker and Uwe Völker. A Comprehensive View on the Human Antibody Repertoire Against Staphylococcus aureus Antigens in the General Population.Front. Immunol., 10 March 2021
https://doi.org/10.3389/fimmu.2021.651619
Etyene Schnurr,Pune N. Paqué,Thomas Attin,Paolo Nann,Jonas Grossmann,Silva Holtfreter,Barbara M. Bröker, Christian Kohler, Binh An Diep, Apoena de Aguiar Ribeiro and Thomas Thurnheer. Staphylococcus aureus Interferes with Streptococci Spatial Distribution and with Protein Expression of Species within a Polymicrobial Oral Biofilm. Antibiotics 2021, 10(2), 116; https://doi.org/10.3390/antibiotics10020116
2020
M. Hussain, C. Kohler, K. Becker. Role of SrtA in pathogenicity of Staphylococcus lugdunensis. Microorganisms 2020, 8(12), 1962; https://doi.org/10.3390/microorganisms8121975
Wagner GE, Föderl-Höbenreich E, Assig K, Lipp M, Berner A, Kohler C, Lichtenegger S, Stiehler J, Karoonboonyanan W, Thanapattarapairoj N, Promkong C, Koosakulnirand S, Chaichana P, Ehricht R, Gad AM, Söffing HH, Dunachie SJ, Chantratita N, Steinmetz I. Melioidosis DS rapid test: A standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection.
PLoS Negl Trop Dis. 2020 Jul 13;14(7) PMID: 32658917
Wolff M, Handtke S, Palankar R, Wesche J, Kohler TP, Kohler C, Gruel Y, Hammerschmidt S, Greinacher A. Activated platelets kill Staphylococcus aureus, but not Streptococcus pneumoniae-The role of FcγRIIa and platelet factor 4/heparinantibodies. J Thromb Haemost. 2020 Apr 1. doi: 10.1111/jth.14814. [Epub ahead of print]
2019
Trinh TT, Assig K, Tran QTL, Goehler A, Bui LNH, Wiede C, Folli B, Lichtenegger S, Nguyen TT, Wagner GE, Kohler C, Steinmetz I. Erythritol as a single carbon source improves cultural isolation of Burkholderia pseudomallei from rice paddy soils. PLoS Negl Trop Dis. 2019 Oct 21;13(10):e0007821. doi: 10.1371/journal.pntd.0007821.
Kohler C, Proctor RA, Bayer AS, Yeaman MR, Lalk M, Engelmann S, Mishra NN. Proteomic and Membrane Lipid Correlates of Reduced Host Defense Peptide. Antibiotics (Basel). 2019 Sep 28;8(4). pii: E169. doi: 10.3390/antibiotics8040169.
Scheuch M, Freiin von Rheinbaben S, Kabisch A, Engeßer J, Ahrendt S, Dabers T, Kohler C, Holtfreter S, Bröker BM, Stracke S. Staphylococcus aureus colonization in hemodialysis patients: a prospective 25 months observational study. BMC Nephrol. 2019 May 6;20(1):153. doi: 10.1186/s12882-019-1332-z.
Langner I, Kramer A, Matthes R, Rebert F, Kohler C, Koban I, Hübner NO, Kohlmann T, Patrzyk M. Inhibition of microbial growth by cold atmospheric plasma compared with the antiseptics chlorhexidine digluconate, octenidine dihydrochloride and polyhexanide. Plasma Process Polym. 2019;16:e1800162 https://doi.org/10.1002/ppap.201800162
2018
Duong Tuan Linh, Sandra Schwarz, Harald Gross, Katrin Breitbach, Falko Hochgräfe, Jörg Mosterz, Kristin Eske-Pogodda, Gabriel E. Wagner, Ivo Steinmetz, Christian Kohler. GvmR - a novel LysR-type regulator involved in virulence and primary and secondary metabolism of Burkholderia pseudomallei. Front Microbiol. 2018 May 16;9:935. doi: 10.3389/fmicb.2018.00935.
2017
Göhler A, Trung TT, Hopf V, Kohler C, Hartleib J, Wuthiekanun V, Peacock SJ, Limmathurotsakul D, Tuanyok A, Steinmetz I. Multitarget Quantitative PCR Improves Detection and Predicts Cultivability of the Pathogen Burkholderia pseudomallei. Appl Environ Microbiol. 2017;83(8)
Whiteley L, Meffert T, Haug M, Weidenmaier C, Hopf V, Bitschar K, Schittek B, Kohler C, Steinmetz I, West TE, Schwarz S. Entry, Intracellular Survival, and Multinucleated-Giant-Cell-Forming Activity of Burkholderia pseudomallei in Human Primary Phagocytic and Nonphagocytic Cells. Infect Immun. 2017;85(10).
Obasi A, Nwachukwu S, Ugoji E, Kohler C, Göhler A, Balau V, Pfeifer Y, Steinmetz I. Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae from Pharmaceutical Wastewaters in South-Western Nigeria. Microb Drug Resist. 2017. doi: 10.108910.1089/mdr.2016.0269
2016
Kohler C, Dunachie SJ, Müller E, Kohler A, Jenjaroen K, Teparrukkul P, Baier V, Ehricht R, Steinmetz I. Rapid and Sensitive Multiplex Detection of Burkholderia pseudomallei-Specific Antibodies in Melioidosis Patients Based on a Protein Microarray Approach. PLoS Negl Trop Dis. 2016;10(7):e0004847
Sakellariou C, Gürntke S, Steinmetz I, Kohler C, Pfeifer Y, Gastmeier P, Schwab F, Kola A, Deja M, Leistner R. Sepsis Caused by Extended-Spectrum Beta-Lactamase (ESBL)-Positive K. pneumoniae and E. coli: Comparison of Severity of Sepsis, Delay of Anti-Infective Therapy and ESBL Genotype. PLoS One. 2016;11(7):e0158039.
Kohler C , Gierok P, Steinmetz I, Lalk M. Burkholderia pseudomallei Colony Morphotypes Show a Synchronized Metabolic Pattern after Acute Infection. PLoS Negl Trop Dis. 2016 4;10(3):e0004483.
2015
Werten S, Kohler C, Bayer NJ, Steinmetz I, Hinrichs W. Structural analysis and knock-out of a Burkholderia pseudomallei homolog of the eukaryotic transcription coactivator PC4. Gene. 2016;577(2):140-7.
Kloppot P, Selle M, Kohler C, Stentzel S, Fuchs S, Liebscher V, Müller E, KaleD, Ohlsen K, Bröker BM, Zipfel PF, Kahl BC, Ehricht R, Hecker M, Engelmann S. Microarray-based identification of human antibodies against Staphylococcus aureus antigens. Proteomics Clin Appl. 2015;9(11-12):1003-11.
Kohler C, Lourenço RF, Bernhardt J, Albrecht D, Schüler J, Hecker M, Gomes SL. A comprehensive genomic, transcriptomic and proteomic analysis of a hyperosmotic stress sensitive α-proteobacterium. BMC Microbiol. 2015;15:71.
2014
Gürntke S, Kohler C, Steinmetz I, Pfeifer Y, Eller C, Gastmeier P, Schwab F, Leistner R. Molecular epidemiology of extended-spectrum beta-lactamase(ESBL)-positive Klebsiella pneumoniae from bloodstream infections and risk factors for mortality. J Infect Chemother. 2014;20(12):817-9.
Leistner R, Sakellariou C, Gürntke S, Kola A, Steinmetz I, Kohler C, PfeiferY, Eller C, Gastmeier P, Schwab F. Mortality and molecular epidemiology associated with extended-spectrum β-lactamase production in Escherichia coli from bloodstream infection. Infect Drug Resist. 2014;7:57-62.
2012
Kola A, Kohler C, Pfeifer Y, Schwab F, Kühn K, Schulz K, Balau V, Breitbach K, Bast A, Witte W, Gastmeier P, Steinmetz I. High prevalence of extended-spectrum-β-lactamase-producing Enterobacteriaceae in organic and conventional retail chicken meat, Germany. J Antimicrob Chemother. 2012;67(11):2631-4.
Kohler C, Lourenço RF, Avelar GM, Gomes SL. Extracytoplasmic function (ECF) sigma factor σF is involved in Caulobacter crescentus response to heavy metal stress. BMC Microbiol. 2012;12:210.
2011
Kusch K, Hanke K, Holtfreter S, Schmudde M, Kohler C, Erck C, Wehland J, Hecker M, Ohlsen K, Bröker B, Engelmann S. The influence of SaeRS and σ(B) on the expression of superantigens in different Staphylococcus aureus isolates. Int J Med Microbiol. 2011;301(6):488-99.
Lourenço RF, Kohler C, Gomes SL. A two-component system, an anti-sigma factor and two paralogous ECF sigma factors are involved in the control of general stress response in Caulobacter crescentus. Mol Microbiol. 2011;80(6):1598-612.
2010
Pagels M, Fuchs S, Pané-Farré J, Kohler C, Menschner L, Hecker M, McNamarra PJ, Bauer MC, von Wachenfeldt C, Liebeke M, Lalk M, Sander G, von Eiff C, Proctor RA, Engelmann S. Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus. Mol Microbiol. 2010 1;76(5):1142-61.
Quiel A, Jürgen B, Piechotta G, Le Foll AP, Ziebandt AK, Kohler C, Köster D, Engelmann S, Erck C, Hintsche R, Wehland J, Hecker M, Schweder T. Electrical protein array chips for the detection of staphylococcal virulence factors. Appl Microbiol Biotechnol. 2010;85(5):1619-27.
2009
Eichstaedt S, Gäbler K, Below S, Müller C, Kohler C, Engelmann S, Hildebrandt P, Völker U, Hecker M, Hildebrandt JP. Effects of Staphylococcus aureus-hemolysin A on calcium signalling in immortalized human airway epithelial cells. Cell Calcium. 2009;45(2):165-76.
Below S, Konkel A, Zeeck C, Müller C, Kohler C, Engelmann S, Hildebrandt JP. Virulence factors of Staphylococcus aureus induce Erk-MAP kinase activation and c-Fos expression in S9 and 16HBE14o- human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2009;296(3):L470-9.
2008
Kohler C, von Eiff C, Liebeke M, McNamara PJ, Lalk M, Proctor RA, Hecker M, Engelmann S. A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus. J Bacteriol. 2008;190(19):6351-64.
Grumann D, Scharf SS, Holtfreter S, Kohler C, Steil L, Engelmann S, Hecker M, Völker U, Bröker BM. Immune cell activation by enterotoxin gene cluster (egc)-encoded and non-egc superantigens from Staphylococcus aureus. J Immunol. 2008;181(7):5054-61.
2007
Makhlin J, Kofman T, Borovok I, Kohler C, Engelmann S, Cohen G, Aharonowitz Y. Staphylococcus aureus ArcR controls expression of the arginine deiminase operon. J Bacteriol. 2007;189(16):5976-86.
Fuchs S, Pané-Farré J, Kohler C, Hecker M, Engelmann S. Anaerobic gene expression in Staphylococcus aureus. J Bacteriol. 2007;189(11):4275-89.
2006
Holtfreter S, Roschack K, Eichler P, Eske K, Holtfreter B, Kohler C, Engelmann S, Hecker M, Greinacher A, Broker BM. Staphylococcus aureus carriers neutralize superantigens by antibodies specific for their colonizing strain: a potential explanation for their improved prognosis in severe sepsis. J Infect Dis. 2006;193(9):1275-8.
2005
Kohler C, Wolff S, Albrecht D, Fuchs S, Becher D, Büttner K, Engelmann S, Hecker M. Proteome analyses of Staphylococcus aureus in growing and non-growing cells: a physiological approach. Int J Med Microbiol. 2005 c;295(8):547-65.
2003
Kohler C, von Eiff C, Peters G, Proctor RA, Hecker M, Engelmann S.
Physiological characterization of a heme-deficient mutant of Staphylococcus aureus by a proteomic approach. J Bacteriol. 2003;185(23):6928-37.
