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Our imaging equipment:
- DeltavisionRT Image restoration workstation
- Microscope Base: Olympus IX71 w/ Nomarski Differential Interference Contrast (DIC) in Transmitted Light
- Vincent Associates Uniblitz Shutters VS25
- Objectives: UPlanApo 100x/1.35 Oil; UPlanApo 60x/1.40 Oil, 40x/Oil
- Camera: Photometrics CoolSNAP HQ
- Light Source: OSRAM HBO 100W/2, Halogen, Lasers
- Temperature Controlled Growth: Solent Scientific
- Software: SoftWorx Explorer Suite & Bitplane Imaris 4.0
- Filters and dichroics from AHF or Chroma:
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| Dye | Excitation | Emission | Compatible dye |
| DAPI | EX 360/40 | EM 457/50 | Hoechst 34850 |
| CFP | EX 436/10 | EM 470/30 | |
| FITC | EX 490/20 | EM 528/38 | GFP |
| YFP | EX 500/20 | EM 535/30 | |
| TRITC | EX 555/28 | EM 617/73 | RFP |
| CY5 | EX 640/20 | EM 685/40 | Alexa 647 |
- Zeiss LSM 510 META Laser scanning confocal microscope
- Microscope Base: Axiovert 200M w/ Differential Interference Contrast (DIC)
- Objectives: 100x/1.40 Oil; 63x/1.2 Water, 40x, 20x
- 2 PMT, 1 spectral META Detektor (32 channel Hamamatsu PMT array)
- Light Source:
- 458/477/488/514 nm Argon gas laser
- 405 nm diode laser
- 561 nm diode laser
- 633 nm diode laser
- Environmental Control (CO2, T): PECON
- Software: Zeiss LSM Software release 3.5 plus several modules (Kinetic, FRET, FRAP, 3D)
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