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// Functional Genomics and Physiology of S. aureus | |||||||||||||||||||||||||||
A1: Physiological proteomics of Staphylococcus aureus - from stress physiology to in vivo proteomics[ Project completed ]The main goal of the project is to use functional genomics technologies, particularly proteomics, to gain new information on the cellular physiology and virulence of S. aureus.
The project is based on the progress made in the first funding period, particularly on the identification and quantification of the entire proteome. The dynamics of the protein inventory, including the protein quality-control system in response to infection-related stimuli, the role of crucial regulons in metabolism and pathogenicity, and some aspects of in vivo proteomics (infection-related conditions) will be analyzed.
Contact Prof. Dr. Michael Hecker Institut für Mikrobiologie Ernst-Moritz-Arndt-Universität Greifswald Friedrich-Ludwig-Jahn-Straße 15 D-17487 Greifswald Phone +49 3834 864233 Fax +49 3834 864202 E-Mail: hecker@uni-greifswald.de
Dr. Susanne Engelmann Institut für Mikrobiologie Ernst-Moritz-Arndt-Universität Greifswald Friedrich-Ludwig-Jahn-Straße 15 D-17487 Greifswald Phone +49 3834 864227 Fax +49 3834 864202 E-Mail: Susanne.Engelmann@uni-greifswald.de
A2: Phosphoproteomic analysis of Staphylococcus aureus: Functional characterization of kinases and identification of their substratesReversible protein phosphorylation is a common regulatory mechanism in all living organisms, from bacteria to humans. In this project, the function of the eukaryotic-like serine/threonine kinase (ESTK) PknB and its corresponding phosphatase Stp in Staphylococcus aureus is studied.
In particular, we are interested in signals activating PknB/Stp activity, signal transduction processes, and their role in physiology, virulence, and antibiotic resistance.
Contact PD Dr. Knut Ohlsen Institut für Molekulare Infektionsbiologie Julius-Maximilians-Universität Würzburg Josef-Schneider-Str. 2, Bau D15 D-97080 Würzburg Phone +49 931 3182155 Fax +49 931 3182578 E-Mail: knut.ohlsen@mail.uni-wuerzburg.de
A3: Impact of membrane potential on Staphylococcus aureus biofilmsThe research topics of A3 have always been centered on staphylococcal physiology and biofilm survival strategies. In the first funding period, the focus was on the physiology of Staphylococcus aureus biofilm; in the second period, it was on generating a membrane potential and regulating nitrate respiration; in the third period, the focus will be on a novel membrane potential-generating system in S. aureus and its impact on biofilm physiology.
Particular attention will be paid to “physiological hotspots” in S. aureus biofilms resulting from spatiotemporal changes in expression of genes of energy metabolism.
Contact Prof. Dr. Friedrich Götz LS Mikrobielle Genetik Eberhard-Karls-Universität Tübingen Auf der Morgenstelle 28 D-72076 Tübingen Phone +49 7071 2974635 or -36 Fax +49 7071 295937 E-Mail: friedrich.goetz@uni-tuebingen.de
Prof. Dr. Katharina Riedel Institut für Mikrobiologie Ernst-Moritz-Arndt-Universität Greifswald Friedrich-Ludwig-Jahn-Strße 15, D-17487 Greifswald Phone: +49 3834 86 4200 E-Mail: riedela@uni-greifswald.de
A6: X-ray analysis of enzymes and virulence factors of pathogenic staphylococciWe propose to determine three-dimensional structures of selected staphylococcal proteins in order to enhance understanding of their mechanism of function and develop compounds that modulate their function, for example by interfering with ligand binding. Our goals for the next funding period are to build on work performed during the last funding period and provide a structural foundation for the interaction of several staphylococcal proteins with cognate ligands.
The congruency of the proposed research is to establish a foundation for a better understanding of key metabolic and transport processes of staphylococci, and eventually use this information for improved therapy.
Contact Prof. Dr. Thilo Stehle Interfakultäres Institut für Biochemie Eberhard-Karls- Universität Tübingen Hoppe-Seyler-Str. 4 D-72076 Tübingen Phone +49 7071 2973043 Fax +49 7071 295565 E-Mail: thilo.stehle@uni-tuebingen.de
A8: A systems biology perspective of regulatory and metabolic adaptation of Staphylococcus aureus to infection-related conditionsPrevious work has shown that S. aureus responds rapidly and effectively to infection-relevant stimuli by adaptations of its gene expression and reorganization of protein complexes and metabolic pathways. For a comprehensive understanding of S. aureus physiology and virulence, we will now elucidate adaptation of functional interaction networks with a focus on host challenges by a combination of experimental, bioinformatics and systems biology approaches.
This reference model transcends the idea of static protein complexes by integrating the full dynamics of the regulatory and metabolic networks in S. aureus and will expand existing S. aureus pathogen models.
Contact Dr. Jan Pané-Farré Institut für Mikrobiologie Ernst-Moritz-Arndt-Universität Greifswald Friedrich-Ludwig-Jahn-Straße 15 D-17487 Greifswald Phone: 03834-86 4237 E-Mail: janpf@uni-greifswald.de
Prof. Dr. Thomas Dandekar Lehrstuhl für Bioinformatik, Biocenter Julius-Maximilians-Universität Würzburg Am Hubland D-97074 Würzburg Phone +49 931 3184551 Fax +49 931 8884552 E-Mail: dandekar@biozentrum.uni-wuerzburg.de
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